Proteomics and Biological Mass Spectrometer Facility
The mission of the facility is to seed proteomics and mass spectrometry technology to research labs at Montana State University and affiliated programs. Periodic hands-on training sessions are offered free of charge. The goal of the training modules is to expand access to researchers with little previous training in proteomics and mass spectrometry.
The facility houses state-of-the-art instrumentation for mass spectrometry and proteomics analysis, which are available on a fee/hour basis. In addition, the facility supports organic synthesis projects through high mass accuracy/high resolution mass spectrometry on a fee-for-service basis. The facility possesses:
- A Bruker Biflex III MALDI-TOF MS with high mass modifications
- A Bruker Esquire 3000 LC-ESI-IonTrap MS/MS system;
- A Bruker MicroTof LC-ESI-TOF MS system that has high sensitivity with mass accuracy greater than 5ppm;
- An Agilent XCT Ultra nanoLC-ESI-IonTrap MS/MS system equipped with the microfluidic ChipLC system;
- A GE Healthcare Typhoon system for fluorescent dye gel imaging;
- Decyder 6.5 gel image analysis software;
- Nonlinear Dynamics SameSpots image analysis software;
- Several IPGphors and large format gel tanks for 2D gels; and
- Mascot and SpectrumMill dedicated servers for data analysis. All ESI-MS instruments are interfaced with Agilent 1100 HPLC modules and autosamplers. In addition, the facility houses a Hewlett-Packard GC/MS system with an autosampler.
The facility has modules for LC-MALDI and simultaneous multiwavelength monitoring and mass spectrometry analysis with microfraction collection available. In the near future, the facility will upgrade the SCTUltra to add ETD to the currently available CID. This technology promises to be a real breakthrough for identification of labile post-translational modifications.
Bioinformatics and Biostatistics Core Director